<span style="font-size:14px"><span style="font-family:Arial,Helvetica,sans-serif">Airborne microbial contamination was collected from the main directions of the holly mosque (Al-Haram mosque), Makkah city, by using the gravitational method. Bacteria, fungi and actinomycete concentrations ranged between 1470&ndash; 21800 CFU/m³; 44 - 572 CFU/m³, and 0.0 - 264 CFU/m³, respectively at all directions. Bacterial concentrations significantly differed between directions, and Gram positive bacteria constituted ~ 90-100% of the total bacterial isolates. Gammaproteobacteria<em> </em>were<em> </em>the common Gram negative bacteria, and <em>Aspergillus </em>was the predominant fungal genera. Mesophilic bacteria negatively related (P&lt; 0.05) with relative humidity. Human activities had more effective influence on the microbial concentrations than the meteorological factors.</span></span>