The current study focuses on chlorpyrifos-degrading bacteria that were isolated from soil samples of Suthamalli Dam, Tirunelveli District, Tamil Nadu. The pesticide were extracted from the sample using the SLE-LTP method. UV-visible spectroscopy showed supreme absorbance at 280 nm, confirming the existence of chlorpyrifos in the soil. Eight bacterial species were isolated for bioremediation on nutrient-agar medium. Three isolates, RFS1, RFS4, and RFS6 (Streptococcus, Rhodococcus and Bacillus species respectively through morphology and biochemical tests) were selected based on their ability to grow in varying concentrations of chlorpyrifos (25, 50, 75, and 100 ul/ml). Chlorpyrifos biodegradation was studied using these bacterial cultures over 12 days. This study conclude that Streptococcus sp, Rhodococcus sp, and Bacillus sp could effectively degrade the chlorpyrifos in contaminated agricultural fields.