<p><span style="font-family:Arial,Helvetica,sans-serif"><span style="font-size:14px">A bacterial strain AST2.2 with chlorpyrifos degrading ability was isolated by enrichment technique from apple orchard soil with previous history of chlorpyrifos use. Based on the morphological, biochemical tests and 16S rRNA sequence analysis, AST2.2 strain was identified as <em>Pseudomonas resinovarans</em>. The strain AST2.2 utilized chlorpyrifos as the sole source of carbon and energy. This strain exhibited growth upto 400mg/l concentration of chlorpyrifos and exhibited high extracellular organophosphorus hydrolase (OPH) activity. Gas chromatography-flame ionization detector (GC-FID) studies revealed that<em> Pseudomonas resinovarans </em>AST2.2 degraded 43.90 % of chlorpyrifos (400 mg/l) within 96 hrs. Intermediates of chlorpyrifos degradation were identified using GC-MS. This strain have potential to degrade chlorpyrifos and thus can be used for bioremediation and ecological restoration of sites contaminated with chlorpyrifos.</span></span></p>