<p>Microalgae chlorella is an organism capable of photosynthesis that is less than 2mm in diameter. The biodiesel extracted from algae using chloroform/methanol extraction solvent system then undergone three different transesterification processes based on three different catalysts <em>viz. </em>Alkali catalyst, Acid catalyst&nbsp; and Enzymatic catalyst with&nbsp; two temperature (50&deg;C and 60 &deg;C) and with 1:5 methanol to bio-oil ratio. After transesterification using different catalysts, the fuel properties were measured. All the properties were compared with standard value of ASTM D 6751 standards. Alkali catalyst yield highest biodiesel (92 %) at 60 &deg;C temperature. Also, the closest value of different fuel properties found at par with standard value of ASTM D 6751 standards <em>viz.</em> moisture content, carbon residue, calorific value, specific gravity, acid value, flash point, viscosity, density, viscosity were found to be 0.01%, 0.04%, 40.41 MJ/kg, 0.83, 0.23 mg KOH/g, 143.67 &deg;C, 5.16 mm²/s, 0.83 g/cm³ respectively in the biodiesel which was yield by transesterification done using Alkali catalyst (0.56 % NaOH) at 60 &deg;C temperature.</p>