Water and energy shortages, has increased the need for methods that can provide low energy for desalination of sea water. Microbial desalination cell is one of the most important of these methods. In this study we use air cathode MDC for desalination of seawater. The maximum voltage, power and current density was 607mV, 521mW/m² and 858mA/m² (25mM PBS) and 701mV, 695mW/m² and 992mA/m² (50mM PBS) respectively. During the period of the voltage generation in 50mM PBS was about 1.5 times of 25mM PBS. Under this situation, EC of seawater with initial  electrical conductivity declined by 48.31±3% (25mM PBS) and 46.71±2.73% (50mM PBS). As well as decrease of salt from sea water in the middle chamber, EC in synthetic wastewater and catholyte slightly increased. So that Change percent of  EC in synthetic wastewater was 44.20 ± 11.94(25mM PBS) and 27.94 ± 3 (50 mM PBS) and in catholyte was 211.66 ± 22.41(25mM PBS) and 119.24 ± 11.25 (50 mM PBS) respectively. These results show that the MDC can also be used as a pretreatment to reverse osmosis; simultaneously the energy required in this process is also partly meet.