Present study was undertaken to assess the spatial and temporal distribution of phytoplanktons with respect to the changes in various physico-chemical parameters of Dongarwada ghat of river Narmada Madhya Pradesh. The study was carried for a year from March 2010 to February 2011.The results revealed presence of total of 27 taxa of phytoplanktons belonging to 4 families were found in order of <em>Chlorophyceae</em> (47%) &gt;<em>Cyanophyceae</em> (27%) &gt;<em>Bacillariophyceae</em> (23%)&gt;<em>Euglenophyceae</em> (3%).Diversity parameters such as Shannon index ranged from 1.092-0.37, Simpson index from 0.6622-0.6202, evenness diversity index ranged from 0.9932 -0.7288 and Margelef index between 0.5459-0.2951.Various physico-chemical parameters of water analyzed depicted a positive correlation with diversity and distribution of Phytoplankton.